The 5-Second Trick For thr77
The 5-Second Trick For thr77
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in lots of tumors, which leads to chromosome instability, and thus might lead to tumorigenesis. This protein was identified to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential part in promoting mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two extra splice variants have been documented but in-depth nucleotide sequence info just isn't nevertheless readily available.
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Blocking peptides are peptides that bind especially on the target antibody and block antibody binding. These peptide generally is made up of the epitope recognized with the antibody.
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This information outlines further achievable optimization of cellular and immunoassay parameters to ensure the best possible results are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs towards the highly conserved cyclin family, whose members are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and is particularly degraded as cells development via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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